Tumorsphere assay provides more accurate prediction of in vivo responses to chemotherapeutics

@article{Kim2013TumorsphereAP,
  title={Tumorsphere assay provides more accurate prediction of in vivo responses to chemotherapeutics},
  author={Soyoung Kim and Caroline M. Alexander},
  journal={Biotechnology Letters},
  year={2013},
  volume={36},
  pages={481-488}
}
Although the sphere culture system has been widely used in stem cell biology, its application for drug screening is limited due to lack of standardized, rapid analytical tools. To optimize sphere cultures for in vitro screening of drugs, we evaluated the properties of primary tumor cells growing as tumorspheres and compared their chemosensitivity to those of cells growing in monolayer. Most cells in tumorsphere cultures were quiescent whereas cells in monolayer culture had a high mitotic index… CONTINUE READING