Tumor-specific monoclonal antibodies for renal cell carcinoma.

  title={Tumor-specific monoclonal antibodies for renal cell carcinoma.},
  author={T Sch{\"a}rfe and Eduard Becht and R Kaltwasser and Joachim W. Th{\"u}roff and G{\"u}nther Heinz Jacobi and Rudolph Hohenfellner},
  journal={European urology},
  volume={11 2},
Monoclonal antibodies, tumor-specific for renal cell carcinoma (RCC), were produced in Balb/C mice, hyperimmunized with tumor cell suspensions from a histological grade II tumor. Boosting with lectin-immobilized tumor-antigen rendered high yields of specific antibody-producing hybrids. Hybridoma supernatants were screened for specificity using a solid-phase enzyme-linked immunoassay. Testing in parallel for reactivity with tumor tissue and corresponding autologous normal kidney material, only… CONTINUE READING