Tumor-promoting phorbol esters promote melanogenesis and prevent expression of the adrenergic phenotype in quail neural crest cells.

@article{SieberBlum1981TumorpromotingPE,
  title={Tumor-promoting phorbol esters promote melanogenesis and prevent expression of the adrenergic phenotype in quail neural crest cells.},
  author={Maya Sieber-Blum and Fritz Sieber},
  journal={Differentiation; research in biological diversity},
  year={1981},
  volume={20 2},
  pages={117-23}
}
Tumor-promoting phorbol esters were used to manipulate the in vitro development of neural crest cells. When plated at clonal density in secondary culture, quail neural crest cells from the trunk region gave rise to three types of colonies, pigmented, unpigmented, and mixed. Pigmented colonies consisted exclusively of melanocytes; up to 50% of the unpigmented mixed colonies contained adrenergic nerve cells which could be identified by a catecholamine-specific histofluorescence method. Addition… CONTINUE READING