Tumor initiating cells: development and critical characterization of a model derived from the A431 carcinoma cell line forming spheres in suspension.

@article{Bortolomai2010TumorIC,
  title={Tumor initiating cells: development and critical characterization of a model derived from the A431 carcinoma cell line forming spheres in suspension.},
  author={Ileana Bortolomai and Silvana Canevari and Ileana Facetti and Loris De Cecco and Giancarlo Castellano and Alberto Zacchetti and Malcolm R Alison and Silvia Miotti},
  journal={Cell cycle},
  year={2010},
  volume={9 6},
  pages={1194-206}
}
To investigate the tumor fraction with cancer stem/tumor initiating cell (CSC/TIC) characteristics, we tested the human cervical carcinoma cell lines A431, Caski and SiHa, by growth as non-adherent spheres in specific media and aldehyde dehydrogenase (ALDH) enzymatic activity. A good correlation between the two parameters was observed and the highest levels were observed in A431 cell line that was selected for characterization of the CSC/TIC fraction. A431 parental cells already displayed… CONTINUE READING