Tumor cell invasion of model 3-dimensional matrices: demonstration of migratory pathways, collagen disruption, and intercellular cooperation.

@article{Horino2001TumorCI,
  title={Tumor cell invasion of model 3-dimensional matrices: demonstration of migratory pathways, collagen disruption, and intercellular cooperation.},
  author={Kai Horino and Andrei L. Kindezelskii and Victor M. Elner and Bret A. Hughes and Howard R. Petty},
  journal={FASEB journal : official publication of the Federation of American Societies for Experimental Biology},
  year={2001},
  volume={15 6},
  pages={932-9}
}
We report a novel 3-dimensional model for visualizing tumor cell migration across a nylon mesh-supported gelatin matrix. To visualize migration across these model barriers, cell proteolytic activity of the pericellular matrix was detected using Bodipy-BSA (fluorescent upon proteolysis) and DQ collagen (fluorescent upon collagenase activity). For 3-dimensional image reconstruction, multiple optical images at sequential z axis positions were deconvoluted by computer analysis. Specificity was… CONTINUE READING