Tumor-associated MUC1 tandem-repeat glycopeptide microarrays to evaluate serum- and monoclonal-antibody specificity.

  title={Tumor-associated MUC1 tandem-repeat glycopeptide microarrays to evaluate serum- and monoclonal-antibody specificity.},
  author={Ulrika Westerlind and Hendrik V Schr{\"o}der and Alexandra Hobel and Nikola Gaidzik and Anton Kaiser and Christof M. Niemeyer and Edgar Schmitt and Herbert Waldmann and Horst Kunz},
  journal={Angewandte Chemie},
  volume={48 44},
Nonspecific nuclear uptake of anti-MUC1 aptamers by dead cells: the role of cell viability monitoring in aptamer targeting of membrane-bound protein cancer biomarkers.
The identification of dead cells and cellular debris using viability stains and the subsequent exclusion of these cells from FACS analysis was identified as an essential requirement for the evaluation of aptamer binding specificity to live cell populations of the cancer cell lines MCF-7, Hs578T and SW480.
Amplified detection of breast cancer autoantibodies using MUC1-based Tn antigen mimics.
The suitability of the sp2-iminosugar glycopeptides to detect anti-MUC1 antibodies was demonstrated and serological experiments showed stage I breast cancer autoantibodies binding with a specific unnatural glycopePTide with almost no healthy serum interaction.
Chemical Synthesis and Immunological Evaluation of Helicobacter pylori Serotype O6 Tridecasaccharide O-Antigen Containing a Unique DD-Heptoglycan.
The development of glycoconjugate vaccines against Helicobacter pylori is challenging. An exact epitope of the H. pylori lipopolysaccharides (LPS) O-antigens that contain Lewis determinant
Positional Scanning MUC1 Glycopeptide Library Reveals the Importance of PDTR Epitope Glycosylation for Lectin Binding
This study synthesized for the first time a MUC1-derived positional scanning synthetic glycopeptide combinatorial library (PS-SGCL) that vary in number and location of cancer-associated Tn antigen using the “tea bag” approach and revealed a carbohydrate density-dependent affinity trend and site-specific glycosylation requirements for high affinity binding to these lectins.
Mucins and Pathogenic Mucin-Like Molecules Are Immunomodulators During Infection and Targets for Diagnostics and Vaccines
The distribution, structure, role as immunomodulators, and the correlation of human mucins with diseases are summarized and a comparative analysis of mucin-like molecules present in human pathogens are performed.
Origins and clinical relevance of proteoforms in pediatric malignancies
As proteome coverage and the ability to detect complex proteoforms increase, the most need for change is in data compilation and database availability to mediate high-level data analysis and allow for better functional annotation of proteoform.
Protective Epitope Discovery and Design of MUC1-based Vaccine for Effective Tumor Protections in Immunotolerant Mice.
The antibodies exhibited remarkable selectivities toward human breast cancer tissues, suggesting its high translational potential, and a peptide array has been synthesized.
Toward Automated Enzymatic Synthesis of Oligosaccharides.
The goal of this survey is to provide a foundation on which continued technological advancements can be made to promote the automated enzymatic synthesis of oligosaccharides.


Chemische Verfahren zur Herstellung von Proteinbiochips
Proteinbiochips sind integrale Bestandteile einer wachsenden Zahl medizinischer und bioanalytischer Anwendungen, weshalb das Interesse an Methoden zur Proteinimmobilisierung fur die Herstellung
Die Herstellung von DNA‐Chips
Die Entwicklung von Mikroarrays gehort derzeit zu den aktuellsten Gebieten der biologischen Forschung. Sie werden meistens fur die Analyse von Nucleinsauren eingesetzt, insbesondere um zu beurteilen,
Robust immune responses elicited by a fully synthetic three-component vaccine.
It is found that a three-component vaccine composed of a TLR2 agonist, a promiscuous peptide T-helper epitope and a tumor- associated glycopeptide can elicit in mice exceptionally high titers of IgG antibodies that can recognize cancer cells expressing the tumor-associated carbohydrate.
MUC1 and cancer.
Protein chip technology.
Peptide microarrays: next generation biochips for detection, diagnostics and high-throughput screening.
This review will chart the progress made in peptide microarrays, with a focus on the recent advances that could impact how the field will be shaped in the coming years.