Tumor angiogenic factor. Purification from the Walker 256 rat tumor.


We report here the purification of an angiogenic substance from the Walker 256 rat ascites tumor which is mitogenic for fetal bovine aortic endothelial cells in culture and which also stimulates new blood vessel growth in vivo. Purification was monitored in endothelial cell cultures by cell counting or by [3H]thymidine incorporation into DNA. Lyophilized… (More)


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