Tubulin-blocked state of VDAC studied by polymer and ATP partitioning.

  title={Tubulin-blocked state of VDAC studied by polymer and ATP partitioning.},
  author={Philip A. Gurnev and Tatiana K. Rostovtseva and Sergey M. Bezrukov},
  journal={FEBS letters},
  volume={585 14},
Recently reported functional interaction between voltage-dependent anion channel of the outer mitochondrial membrane, VDAC, and dimeric tubulin is observed as a reversible channel blockage. Using partitioning of poly-(ethylene glycol)s of different molecular weights and reversal potential measurements, we probe the size and ion selectivity of the fully open and tubulin-blocked states of VDAC reconstituted into planar lipid bilayers. While the effective radius of the channel decreases by only a… CONTINUE READING