Tubular Cell HIV-1 gp120 Expression Induces Caspase 8 Activation and Apoptosis

@article{Vashistha2009TubularCH,
  title={Tubular Cell HIV-1 gp120 Expression Induces Caspase 8 Activation and Apoptosis},
  author={Himanshu Vashistha and Mohammed Amir Husain and Dileep Kumar and Pravin C. Singhal},
  journal={Renal Failure},
  year={2009},
  volume={31},
  pages={303 - 312}
}
Renal biopsy data indicate that tubular epithelial cells serve as a reservoir for HIV-1 infection. We studied the effect of HIV-1 gp120 envelope gene expression on tubular cell apoptosis. HIV-1 gp120 was expressed in a lentiviral vector pHR-CMV-IRES2-EGFP-ΔB. This plasmid construct was used to produce pseudotyped virus using VSV-G envelope to enhance the tropism for efficient viral transduction. Human proximal tubular (HK-2) cells were transduced and assayed for cellular injury by trypan blue… 
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