Tuberculosis diagnosis and multidrug resistance testing by direct sputum culture in selective broth without decontamination or centrifugation.

@article{Grandjean2008TuberculosisDA,
  title={Tuberculosis diagnosis and multidrug resistance testing by direct sputum culture in selective broth without decontamination or centrifugation.},
  author={Louis Grandjean and Laura Rodriguez Martin and Robert H. Gilman and Teresa R Valencia and Beatriz S{\'a}nchez Herrera and Willi Quino and Eric J. Ramos and Maribel Rivero and Rub{\'e}n Bugar{\'i}n Montoya and Adrian Roderick Escombe and David D. Coleman and Denis Anthony Mitchison and Carlton A Evans},
  journal={Journal of clinical microbiology},
  year={2008},
  volume={46 7},
  pages={
          2339-44
        }
}
Tuberculosis culture usually requires sputum decontamination and centrifugation to prevent cultures from being overgrown by contaminating bacteria and fungi. However, decontamination destroys many tuberculous bacilli, and centrifugation often is not possible in resource-poor settings. We therefore assessed the performance of Mycobacterium tuberculosis culture with unprocessed samples plated directly by using tuberculosis-selective media and compared this procedure to conventional culture using… CONTINUE READING
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