Tryptophan 232 within OxidosqualeneLanosterol Cyclase from <italic>Saccharomyces </italic><italic>c</italic><italic>erevisiae</italic> Influences Rearrangement and Deprotonation but Not Cyclization Reactions

Abstract

Oxidosqualene−lanosterol cyclases convert oxidosqualene to lanosterol in yeast and mammals. Site-saturated mutants’ construction of Saccharomyces cerevisiae oxidosqualene−lanosterol cyclase, at Trp232 exchanges against proteinogenic amino acids, and product profiles are shown. All mutants, except Lys and Arg, produced protosta-12,24-dien-3â-ol, lanosterol… (More)

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