Tryptic peptides of human thyroglobulin: I. Immunoreactivity with murine monoclonal antibodies.

@article{Saboori1994TrypticPO,
  title={Tryptic peptides of human thyroglobulin: I. Immunoreactivity with murine monoclonal antibodies.},
  author={Ali M. Saboori and C. Lynne Burek and Noel R. Rose and H S Bresler and Monica Vladut Talor and Rudolf C. Kuppers},
  journal={Clinical and experimental immunology},
  year={1994},
  volume={98 3},
  pages={454-8}
}
Human thyroglobulin (Tg) was treated with trypsin at different concentrations of trypsin/Tg for various incubation times at 37 degrees C using non-reducing conditions. A ratio of trypsin to Tg of 1:100 (w/w) was optimal to release small peptides that were reactive to murine MoAbs to human Tg. Most peptides were released after only 1 h incubation with trypsin, but these peptides were further degraded at longer incubation times. However, a few small peptides, the largest of which with an apparent… CONTINUE READING