Trypsin-like activity of membrane-bound midgut proteases from Anticarsia gemmatalis (Lepidoptera: Noctuidae)

@inproceedings{Xavier2005TrypsinlikeAO,
  title={Trypsin-like activity of membrane-bound midgut proteases from Anticarsia gemmatalis (Lepidoptera: Noctuidae)},
  author={Luciana P Xavier and Maria Goreti Almeida Oliveira and Raul Narciso Carvalho Guedes and Agenor Valarades Santos and Salvatore Giovanni De Simone},
  year={2005}
}
Membrane-bound proteases from preparations of the midgut of 5 instar velvetbean caterpillars, Anticarsia gemmatalis (Hübner) were obtained by resuspension of the pellet obtained after 100,000 g centrifugation. As expected of trypsin-like proteases, they hydrolyzed casein and the synthetic substrates N-benzoyl-L-Arg-p-nitroanilidine (L-BApNA) and N-p-tosyl-L-Arg methyl ester (L-TAME). Higher activities were observed at 50°C, and at pH 8.5 and 8.0 for both synthetic substrates L-BApNA and L-TAME… CONTINUE READING

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