Trypsin inhibitor from Poecilanthe parviflora seeds: purification, characterization, and activity against pest proteases.

@article{Garcia2004TrypsinIF,
  title={Trypsin inhibitor from Poecilanthe parviflora seeds: purification, characterization, and activity against pest proteases.},
  author={Viviane Alves Garcia and Maria das Graças Machado Freire and Jos{\'e} Camillo Novello and S{\'e}rgio Marangoni and Maria Macedo},
  journal={The protein journal},
  year={2004},
  volume={23 5},
  pages={343-50}
}
Plants synthesize a variety of molecules, including proteinaceous proteinase inhibitors, to defend themselves of being attacked by insects. In this work, a novel trypsin inhibitor (PPTI) was purified from the seeds of the native Brazilian tree Poecilanthe parviflora (Benth) (Papilioinodeae, Leguminosae) by gel filtration chromatography on a Sephadex G-100 followed by Superdex G75 chromatography (FPLC), Sepharose 4B-Trypsin column, and fractionated by reversed-phase HPLC on a C-18 column. SDS… CONTINUE READING