Trypanosoma rangeli protein tyrosine phosphatase is associated with the parasite's flagellum.


Protein tyrosine phosphatases (PTPs) play an essential role in the regulation of cell differentiation in pathogenic trypanosomatids. In this study, we describe a PTP expressed by the non-pathogenic protozoan Trypanosoma rangeli (TrPTP2). The gene for this PTP is orthologous to the T. brucei TbPTP1 and Trypanosoma cruzi (TcPTP2) genes. Cloning and expression of the TrPTP2 and TcPTP2 proteins allowed anti-PTP2 monoclonal antibodies to be generated in BALB/c mice. When expressed by T. rangeli epimastigotes and trypomastigotes, native TrPTP2 is detected as a ~65 kDa protein associated with the parasite's flagellum. Given that the flagellum is an important structure for cell differentiation in trypanosomatids, the presence of a protein responsible for tyrosine dephosphorylation in the T. rangeli flagellum could represent an interesting mechanism of regulation in this structure.

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@article{Prestes2012TrypanosomaRP, title={Trypanosoma rangeli protein tyrosine phosphatase is associated with the parasite's flagellum.}, author={Elisa Beatriz Prestes and Ethel Bayer-Santos and Patr{\'i}cia Hermes Stoco and Tha{\'i}s Cristine Marques Sincero and Glauber Wagner and Adriana Castilhos Souza Umaki and Stenio Perdig{\~a}o Fragoso and Juliano Bordignon and M{\'a}rio Steindel and Edmundo C. Grisard}, journal={Memórias do Instituto Oswaldo Cruz}, year={2012}, volume={107 6}, pages={713-9} }