Trypanosoma evansi: a convenient model for studying intracellular Ca(2+) homeostasis using fluorometric ratio imaging from single parasites.

@article{Mendoza2001TrypanosomaEA,
  title={Trypanosoma evansi: a convenient model for studying intracellular Ca(2+) homeostasis using fluorometric ratio imaging from single parasites.},
  author={Marta Mendoza and A. Santamarina Mijares and H{\'e}ctor Rojas and Muneer Ramos and Reinaldo Dipolo},
  journal={Experimental parasitology},
  year={2001},
  volume={99 4},
  pages={213-9}
}
The aim of this work was to measure, for the first time, the basal cytosolic Ca(2+) levels of Trypanosoma evansi and to explore the possibility of observing changes in the intracellular Ca(2+) concentration ([Ca(2+)](i)) using fluorescence ratio imaging techniques in single isolated parasites of this species. Under appropriate loading conditions, the high intracellular levels of the Ca(2+) fluorescence probe Fura-2 permits resolution, in real time, of single parasite [Ca(2+)](i) signals… CONTINUE READING

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