Trypanosoma cruzi has not lost its S-adenosylmethionine decarboxylase: characterization of the gene and the encoded enzyme.

@article{Persson1998TrypanosomaCH,
  title={Trypanosoma cruzi has not lost its S-adenosylmethionine decarboxylase: characterization of the gene and the encoded enzyme.},
  author={Karina Persson and Lena {\AA}slund and Birgitta Grahn and Jens Hanke and Olle Heby},
  journal={The Biochemical journal},
  year={1998},
  volume={333 ( Pt 3)},
  pages={527-37}
}
All attempts to identify ornithine decarboxylase in the human pathogen Trypanosoma cruzi have failed. The parasites have instead been assumed to depend on putrescine uptake and S-adenosylmethionine decarboxylase (AdoMetDC) for their synthesis of the polyamines spermidine and spermine. We have now identified the gene encoding AdoMetDC in T. cruzi by PCR cloning, with degenerate primers corresponding to conserved amino acid sequences in AdoMetDC proteins of other trypanosomatids. The amplified… CONTINUE READING