Trypanosoma cruzi: isolation and characterization of aspartyl proteases.

@article{Pinho2009TrypanosomaCI,
  title={Trypanosoma cruzi: isolation and characterization of aspartyl proteases.},
  author={Rosa Pinho and Leila Maria Beltramini and Carlos Roberto Alves and Salvatore Giovanni De-Simone},
  journal={Experimental parasitology},
  year={2009},
  volume={122 2},
  pages={128-33}
}
Two aspartyl proteases activities were identified and isolated from Trypanosoma cruzi epimastigotes: cruzipsin-I (CZP-I) and cruzipsin-II (CZP-II). One was isolated from a soluble fraction (CZP-II) and the other was solubilized with 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CZP-I). The molecular mass of both proteases was estimated to be 120 kDa by HPLC gel filtration and the activity of the enzymes was detected in a doublet of bands (56 and 48 kDa) by substrate-sodium dodecyl… CONTINUE READING

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