Trypanosoma brucei Metacaspase 4 Is a Pseudopeptidase and a Virulence Factor*

@inproceedings{Proto2011TrypanosomaBM,
  title={Trypanosoma brucei Metacaspase 4 Is a Pseudopeptidase and a Virulence Factor*},
  author={William R. Proto and Esther Castanys-Munoz and Alana Black and Laurence Tetley and Catherine X. Moss and Luiz Juliano and Graham H. Coombs and Jeremy C Mottram},
  booktitle={The Journal of biological chemistry},
  year={2011}
}
Metacaspases are caspase family cysteine peptidases found in plants, fungi, and protozoa but not mammals. Trypanosoma brucei is unusual in having five metacaspases (MCA1-MCA5), of which MCA1 and MCA4 have active site substitutions, making them possible non-enzymatic homologues. Here we demonstrate that recombinant MCA4 lacks detectable peptidase activity despite maintaining a functional peptidase structure. MCA4 is expressed primarily in the bloodstream form of the parasite and associates with… CONTINUE READING