Trypanosoma brucei: in vitro propagation of metacyclic forms derived from the salivary glands of Glossina morsitans.

Abstract

1 Metacyclic forms of Trypanosoma brucei obtained from the salivary glands of the tsetse fly, Glossina morsitans have been cultured for the first time in their infective forms for more than 200 days in continuous culture. The parasites were grown at 25 C and 30 C on a bovine embryonic spleen (BESP) feeder layer in buffered RPMI 1640 medium supplemented with… (More)

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Cite this paper

@article{Nyindo1979TrypanosomaBI, title={Trypanosoma brucei: in vitro propagation of metacyclic forms derived from the salivary glands of Glossina morsitans.}, author={Mramba B A Nyindo and N Patel and Nilesh Darji and Taniya Golder}, journal={The Journal of parasitology}, year={1979}, volume={65 5}, pages={751-5} }