Trypanosoma Cruzi Flagellar Repetitive Antigen Expression by Recombinant Baculovirus: Towards an Improved Diagnostics Reagent for Chagas' Disease

Abstract

We constructed a recombinant baculovirus that expressed part of a Trypanosorna cruzi flagellar repetitive antigen (FRA). Both cell- associated and secreted forms of recombinant FRA were detected in cultures of virus-infected Spodoptera frugiperda (Sf9) cells. These forms show a complex pattern after polyacrylamide gel electrophoresis and Western blot… (More)
DOI: 10.1038/nbt1192-1474

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