Truncation of amidated fragment 33-61 of bovine alpha-hemoglobin: effects on the structure and anticandidal activity.

@article{Machado2007TruncationOA,
  title={Truncation of amidated fragment 33-61 of bovine alpha-hemoglobin: effects on the structure and anticandidal activity.},
  author={Alessandra Machado and Mauricio Luis Sforça and Antonio Miranda and Sirlei Daffre and Thelma A. Pertinhez and Alberto Spisni and Maria Ter{\^e}sa Machini Miranda},
  journal={Biopolymers},
  year={2007},
  volume={88 3},
  pages={
          413-26
        }
}
Peptides derived from endogenous hemoglobin play important biological roles in a variety of living systems. In previous works we showed that the fragment 33-61 of bovine alpha-hemoglobin (Hb33-61) and its C-terminus amidated analogue (Hb33-61a) exhibit antimicrobial activity and we determined the 3D structure of Hb33-61a bound to sodium dodecyl sulfate micelles. Here we report that Hb33-61a is lethal to Candida albicans at 6.25 microM probably through disruption of its plasma membrane. In… Expand
Hb40-61a: Novel analogues help expanding the knowledge on chemistry, properties and candidacidal action of this bovine α-hemoglobin-derived peptide.
TLDR
It is indicated that the central target of Hb40-61a in Candida cells is the plasma membrane and that this synthetic hemocidin should be considered as a potential candidacidal for topic uses. Expand
Purification and characterization of Hb 98–114: A novel hemoglobin-derived antimicrobial peptide from the midgut of Rhipicephalus (Boophilus) microplus
TLDR
A novel antimicrobial peptide from the midgut of fully engorged R. (B.) microplus females, which comprises the amino acids 98-114 of the alpha subunit of bovine hemoglobin, is identified and is the first hemocidin with specific antifungal activity to be characterized. Expand
Isolation and characterization of an antimicrobial peptide from bovine hemoglobin α-subunit
In this study, a novel 18-residue linear antimicrobial peptide derived from the central part of the bovine hemoglobin α-subunit was identified. The peptide was purified by a combination of cationicExpand
Dissection of the Structural Features of a Fungicidal Antibody-Derived Peptide
TLDR
The obtained results suggest that the PPII conformation contributes in characterising the cell penetrating and fungicidal properties of the investigated peptides. Expand
Secreted aspartic peptidases of Candida albicans liberate bactericidal hemocidins from human hemoglobin
TLDR
Findings provide interesting new insights into pathophysiology of human vaginal candidiasis and suggest that C. albicans may be able to compete with the other microorganisms of the same physiological niche using the microbicidal peptides generated from the host protein. Expand
Insights on the structure‐activity relationship of peptides derived from Sticholysin II
TLDR
Two synthetic peptides show diverse haemolytic and candidacidal activity that correlate with distinct orientations in SDS micelles and justify the diverse concentration dependence of their candidACidal activity supposing a different mechanism of action. Expand
Immunological properties of oxygen-transport proteins: hemoglobin, hemocyanin and hemerythrin
TLDR
The true extent of oxygen-transport protein dynamics in innate immunity is evaluated, to impress upon the reader the multi-functionality of these ancient proteins on the basis of their structures. Expand
Synthesis and properties of cyclic gomesin and analogues
TLDR
Because of its high therapeutic index, easy synthesis and lower cost, the [Thr2,6,11,15,d‐Pro9]‐Gm analogue remains the best active Gm‐derived AMP developed so far; nevertheless, its elevated instability in human serum may limit its therapeutic potential. Expand
Chemical synthesis, structure–activity relationship, and properties of shepherin I: a fungicidal peptide enriched in glycine-glycine-histidine motifs
TLDR
Results of Shepherin I (Shep I) shed light on the chemistry, structural requirements for activity, and other properties of candidacidal glycine-rich peptides and show that Shep Ia may have strong potential for use in topical application. Expand
Antimicrobial lipopeptides: in vivo and in vitro synthesis
The non-ribosomally assembled lipopeptides display a number of biological activities, including antibiotic, antitumor, immune-modulating and immune-suppressive activities. This review starts withExpand
...
1
2
3
...

References

SHOWING 1-10 OF 53 REFERENCES
The micelle-bound structure of an antimicrobial peptide derived from the alpha-chain of bovine hemoglobin isolated from the tick Boophilus microplus.
TLDR
It is shown that the peptide Hb33-61a bound to SDS micelles exhibits a nonconventional conformation for an antimicrobial peptide, and is able to disrupt the bacterial membrane of Micrococcus luteus A270. Expand
Role of alpha-helical conformation of histatin-5 in candidacidal activity examined by proline variants.
TLDR
It is suggested that the alpha-helical content of Hsn-5 proline variants does not correlate with the candidacidal activity, which suggests alpha- Helix may not be important for anticandidal activity of H Sn-5. Expand
Broad-spectrum antimicrobial activity of hemoglobin.
TLDR
It has been possible to map specific regions of the human hemoglobin molecule that are responsible for significant antimicrobial activity and it is conceivable that, in addition to its role in oxygen transport, hemoglobin functions as an important multi-defense agent against a wide range of microorganisms. Expand
Structural consequences of carboxyamidation of dermaseptin S3.
TLDR
Although the increased potency in amidated antimicrobial peptides can be attributed to the increased overall positive charge, in this case, amidation has had additional effects beyond modifying the net positive charge that have caused the amidated dermaseptin to be more rigid and more extended than its nonamidated analogue. Expand
How C-terminal carboxyamidation alters the biological activity of peptides from the venom of the eumenine solitary wasp.
TLDR
The diverse biological activity of the two peptides is not simply due to the reduction of the net positive charge, as generally suggested, but also to a structural perturbation of the amphipathic alpha-helix that affects their ability to perturb the cell membrane. Expand
Human hemoglobin-derived peptides exhibit antimicrobial activity: a class of host defense peptides.
TLDR
It is concluded that antimicrobial hemoglobin-derived peptides could be important effectors of the innate immune response killing microbial invaders. Expand
Identification of the bactericidal domain of lactoferrin.
TLDR
The studies suggest this domain is the structural region responsible for the bacterial properties of lactoferrin, having effectiveness against various Gram-negative and Gram-positive bacteria at concentrations between 0.3 microM and 3.0 microM, depending on the target strain. Expand
Lytic activity and structural differences of amphipathic peptides derived from trialysin.
TLDR
The results suggest that while amphipathicity is essential for the lytic activity, the selectivity of the active peptides for specific organisms appears to be associated with the structural features of their N- and C-termini. Expand
Possible role of a PXXP central hinge in the antibacterial activity and membrane interaction of PMAP-23, a member of cathelicidin family.
TLDR
Observations made using biosensor technology enabled us to distinguish between the membrane binding and insertion steps, substantiating a proposed kinetic mode of the peptide-membrane interaction in which PMAP-23 first attaches to the membrane via the N- terminal amphipathic helix, after which bending and/or swiveling of the PXXP motif enables insertion of the C-terminal helix into the lipid bilayer. Expand
Solution structure of termite-derived antimicrobial peptide, spinigerin, as determined in SDS micelle by NMR spectroscopy.
TLDR
The alpha-helical structure and the strong electrostatic attraction between four Lys and three Arg residues in spinigerin and the negatively charged polar head groups of the phospholipids on the membrane surface play important roles in disrupting membrane and subsequent cell death. Expand
...
1
2
3
4
5
...