Truncated human serum albumin retains general anaesthetic binding activity.

  title={Truncated human serum albumin retains general anaesthetic binding activity.},
  author={Renyu Liu and Jinsheng Yang and Chung Eun Ha and Nadhipuram V. Bhagavan and Roderic G. Eckenhoff},
  journal={The Biochemical journal},
  volume={388 Pt 1},
Multiple binding sites for anaesthetics in HSA (human serum albumin) make solution studies difficult to interpret. In the present study, we expressed the wild-type HSA domain 3 (wtHSAd3), a peptide with two known anaesthetic binding sites in a yeast expression system. We also expressed a site-directed mutant of domain 3 (Y411Wd3). The stability and secondary structure of the constructed fragments were determined by HX (hydrogen-tritium exchange) and CD spectroscopy. The binding of two general… 

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