Truncated forms of viral VP2 proteins fused to EGFP assemble into fluorescent parvovirus-like particles

@article{Gilbert2006TruncatedFO,
  title={Truncated forms of viral VP2 proteins fused to EGFP assemble into fluorescent parvovirus-like particles},
  author={L Gilbert and Jouni Toivola and Outi V{\"a}lilehto and Taija Saloniemi and Claire Cunningham and Daniel J. White and Anna R. M{\"a}kel{\"a} and Eila Korhonen and Matti Vuento and Christian Oker-Blom},
  journal={Journal of Nanobiotechnology},
  year={2006},
  volume={4},
  pages={13 - 13}
}
Fluorescence correlation spectroscopy (FCS) monitors random movements of fluorescent molecules in solution, giving information about the number and the size of for example nano-particles. The canine parvovirus VP2 structural protein as well as N-terminal deletion mutants of VP2 (-14, -23, and -40 amino acids) were fused to the C-terminus of the enhanced green fluorescent protein (EGFP). The proteins were produced in insect cells, purified, and analyzed by western blotting, confocal and electron… CONTINUE READING