Trout hepatocyte culture: Isolation and primary culture

  title={Trout hepatocyte culture: Isolation and primary culture},
  author={James E Klaunig and Randall J. Ruch and Peter J. Goldblatt},
  journal={In Vitro Cellular & Developmental Biology},
Rainbow trout (Salmo gairdneri) hepatocytes were isolated using a two-step perfusion through the portal vein. A typical perfusion yielded 2.92×106 liver cells with a mean viability of 96.3%. Hepatocytes comprised 93.4% of the total cell isolate. Survival of hepatocytes in suspension culture was dependent on fetal bovine serum concentration and temperature of incubation. Serum concentrations of 5, 10, and 20% produced the highest survival during primary culture. Hepatocyte survival was in… CONTINUE READING


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