Triton X-114 phase separation in the isolation and purification of mouse liver microsomal membrane proteins.

@article{Mathias2011TritonXP,
  title={Triton X-114 phase separation in the isolation and purification of mouse liver microsomal membrane proteins.},
  author={Rommel A Mathias and Yuan-Shou Chen and E. Kapp and D. Greening and S. Mathivanan and R. Simpson},
  journal={Methods},
  year={2011},
  volume={54 4},
  pages={
          396-406
        }
}
Integral membrane proteins (IMPs) mediate several cellular functions including cell adhesion, ion and nutrient transport, and cell signalling. IMPs are typically hard to isolate and purify due to their hydrophobic nature and low cellular abundance, however, microsomes are small lipid vesicles rich in IMPs, which form spontaneously when cells are mechanically disrupted. In this study, we have employed mouse liver microsomes as a model for optimising a method for IMP isolation and… Expand
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