Triton X-100 pretreatment of LR-white thin sections improves immunofluorescence specificity and intensity.

@article{Ghrebi2007TritonXP,
  title={Triton X-100 pretreatment of LR-white thin sections improves immunofluorescence specificity and intensity.},
  author={Salem S Ghrebi and G Rh Owen and Donald Maxwell Brunette},
  journal={Microscopy research and technique},
  year={2007},
  volume={70 7},
  pages={555-62}
}
The staining of intracellular antigenic sites in postembedded samples is a challenging problem. Deterioration of antigenicity and limited antibody accessibility to the antigen are commonly encountered on account of processing steps. In this study preservation of the antigen was achieved by fixing the tissues with mild fixatives, performing partial dehydration, and embedding in a low crosslinked hydrophilic acrylic resin, LR-White. Permeabilization of cell membranes with Triton X-100 is well… CONTINUE READING

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