Trisomy 7p and malignant transformation of human breast epithelial cells following epidermal growth factor withdrawal.

@article{Briand1996Trisomy7A,
  title={Trisomy 7p and malignant transformation of human breast epithelial cells following epidermal growth factor withdrawal.},
  author={Pascale Briand and Kirsten Vang Nielsen and Mogens Winkel Madsen and Ole William Petersen},
  journal={Cancer research},
  year={1996},
  volume={56 9},
  pages={
          2039-44
        }
}
We have reported previously on the first spontaneously immortalized, nonmalignant human breast epithelial cell line, HMT-3522, which is entirely dependent on exogenous epidermal growth factor (EGF). In passage 118, cells were adapted to grow in medium without EGF and a new growth-transformed subline, HMT-3522/gt-1, was generated and propagated at high growth rate without exogenous EGF (Madsen et al., Cancer Res., 52: 1210-1217, 1992). Here we have used this subline and the continuum of the… CONTINUE READING

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