Triplex protein quantification based on stable isotope labeling by peptide dimethylation applied to cell and tissue lysates.

Abstract

Stable isotope labeling is at present one of the most powerful methods in quantitative proteomics. Stable isotope labeling has been performed at both the protein as well as the peptide level using either metabolic or chemical labeling. Here, we present a straightforward and cost-effective triplex quantification method that is based on stable isotope… (More)
DOI: 10.1002/pmic.200800297

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