Triplex DNA: A new platform for polymerase chain reaction – based biosensor

  title={Triplex DNA: A new platform for polymerase chain reaction – based biosensor},
  author={Yubin Li and Xiangmin Miao and Liansheng Ling},
  booktitle={Scientific reports},
Non-specific PCR amplification and DNA contamination usually accompany with PCR process, to overcome these problems, here we establish a sensor for thrombin by sequence-specific recognition of the PCR product with molecular beacon through triplex formation. Probe A and probe B were designed for the sensor, upon addition of thrombin, two probes hybridized to each other and the probe B was extended in the presence of Klenow Fragment polymerase and dNTPs. The PCR amplification occurred with… CONTINUE READING

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  • Author Contributions L.S.Ling and X.M.Miao designed the experiments. Y.B.Li performed experiments and data analysis. L.S.Ling and Y.B.Li wrote the main text. All authors reviewed the manuscript. Additional Information Competing financial interests: The authors declare no competing financial interest
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