Triplet-state monitoring by fluorescence correlation spectroscopy

@article{Widengren1994TripletstateMB,
  title={Triplet-state monitoring by fluorescence correlation spectroscopy},
  author={Jerker Widengren and Rudolf W. Rigler and {\"U}lo Mets},
  journal={Journal of Fluorescence},
  year={1994},
  volume={4},
  pages={255-258}
}
The effects of high excitation intensities in fluorescence correlation spectroscopy (FCS) in terms of saturation and triplet-state build-up have been studied for the case of Rh6G in aqueous solution. It was found that FCS provides a powerful means for the determination of intersystem crossing and triplet-state depopulation rates of fluorophores in solution. 

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Fluorescence Spectroscopy, Springer-Verlag, Berlin/Heidelberg/New

R. Rigler, J. Widcngren, O. Mets
1992
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Handbook of Fluorescent Probes and Research Chemicals, Molecular Probes

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