Triple transcriptional control of the resuscitation promoting factor 2 (rpf2) gene of Corynebacterium glutamicum by the regulators of acetate metabolism RamA and RamB and the cAMP-dependent regulator GlxR.

@article{Jungwirth2008TripleTC,
  title={Triple transcriptional control of the resuscitation promoting factor 2 (rpf2) gene of Corynebacterium glutamicum by the regulators of acetate metabolism RamA and RamB and the cAMP-dependent regulator GlxR.},
  author={Britta Jungwirth and Denise Emer and Iris Brune and Nicole Hansmeier and Alfred P{\"u}hler and Bernhard J Eikmanns and Andreas Tauch},
  journal={FEMS microbiology letters},
  year={2008},
  volume={281 2},
  pages={190-7}
}
The transcriptional regulators RamA, RamB and GlxR were detected to bind to the promoter region of the resuscitation promoting factor 2 (rpf2) gene involved in growth and culturability of Corynebacterium glutamicum. DNA-binding sites were identified by bioinformatic analysis and verified by electrophoretic mobility shift assays with purified hexahistidyl-tagged proteins. Carbon source-dependent deregulation of rpf2 expression was demonstrated in vivo in ramA and ramB mutants and in a C… CONTINUE READING