Triple helix formation: binding avidity of acridine-conjugated AG motif third strands containing natural, modified and surrogate bases opposed to pyrimidine interruptions in a polypurine target.

@article{Orson1999TripleHF,
  title={Triple helix formation: binding avidity of acridine-conjugated AG motif third strands containing natural, modified and surrogate bases opposed to pyrimidine interruptions in a polypurine target.},
  author={Frank M. Orson and Jan E. Klysik and Donald E. Bergstrom and Brian Ward and Gary Glass and Ping Hua and Berma M. Kinsey},
  journal={Nucleic acids research},
  year={1999},
  volume={27 3},
  pages={810-6}
}
A critical issue for the general application of triple-helix-forming oligonucleotides (TFOs) as modulators of gene expression is the dramatically reduced binding of short TFOs to targets that contain one or two pyrimidines within an otherwise homopurine sequence. Such targets are often found in gene regulatory regions, which represent desirable sites for triple helix formation. Using intercalator-conjugated AG motif TFOs, we compared the efficacy and base selectivity of 13 different bases or… CONTINUE READING