Triple Loss of Function of Protein Phosphatases Type 2C Leads to Partial Constitutive Response to Endogenous Abscisic Acid1[C][W][OA]

@article{Rubio2009TripleLO,
  title={Triple Loss of Function of Protein Phosphatases Type 2C Leads to Partial Constitutive Response to Endogenous Abscisic Acid1[C][W][OA]},
  author={Silvia Rubio and Am{\'e}rico Rodrigues and Angela Saez and Marie B. Dizon and Alexander Gall{\'e} and Tae-Houn Kim and Julia Santiago and Jaume Flexas and Julian I. Schroeder and Pedro L. Rodriguez},
  journal={Plant Physiology},
  year={2009},
  volume={150},
  pages={1345 - 1355}
}
The phytohormone abscisic acid (ABA) is a key regulator of plant growth and development as well as plant responses to situations of decreased water availability. Protein phosphatases type 2C (PP2Cs) from group A, which includes the ABI1/HAB1 and PP2CA branches, are key negative regulators of ABA signaling. Specifically, HAB1, ABI1, ABI2, and PP2CA have been shown to affect both seed and vegetative responses to ABA. To further understand their contribution to ABA signaling and to unravel… 
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TLDR
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TLDR
The results indicate that CaADIP1 and its interacting partner CaRLP1 antagonistically regulate the ABA-dependent defense signalling response to drought stress.
ABI1 and PP2CA Phosphatases Are Negative Regulators of Snf1-Related Protein Kinase1 Signaling in Arabidopsis[C][W]
TLDR
It is demonstrated that two clade A type 2C protein phosphatases, established repressors of the abscisic acid hormonal pathway, interact with the SnRK1 catalytic subunit causing its dephosphorylation and inactivation, strengthening the stress response through the cooperation of two key and complementary pathways.
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TLDR
The results showed that the expression of most ZmPP2C-As were dramatically induced by multiple stresses (drought, salt, and ABA), indicating that these genes may have important roles in response to these stresses.
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TLDR
It is proposed that OsPP2C09-mediated ABA desensitization contributes to root elongation under drought-stress conditions in rice.
A DEAD-box RNA helicase, RH8, is critical for regulation of ABA signalling and the drought stress response via inhibition of PP2CA activity.
TLDR
The data indicate that RH8 and its interacting partner PP2CA modulate the drought stress response via ABA-dependent signalling.
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References

SHOWING 1-10 OF 56 REFERENCES
Enhancement of Abscisic Acid Sensitivity and Reduction of Water Consumption in Arabidopsis by Combined Inactivation of the Protein Phosphatases Type 2C ABI1 and HAB11[W]
TLDR
This work has generated ABA-hypersensitive drought-avoidant mutants by simultaneous inactivation of two negative regulators of ABA signaling, i.e. the protein phosphatases type 2C (PP2Cs) ABI-INSENSITIVE1 (ABI1) and HAB1, and suggests that fine tuning of A BA signaling can be attained through combined action of PP2Cs.
Gain-of-function and loss-of-function phenotypes of the protein phosphatase 2C HAB1 reveal its role as a negative regulator of abscisic acid signalling.
TLDR
It is shown that constitutive expression of HAB1 in Arabidopsis under a cauliflower mosaic virus (CaMV) 35S promoter led to reduced ABA sensitivity both in seeds and vegetative tissues, compared to wild-type plants, providing new genetic evidence on the function of a PP2C in ABA signalling.
ABA-Hypersensitive Germination3 Encodes a Protein Phosphatase 2C (AtPP2CA) That Strongly Regulates Abscisic Acid Signaling during Germination among Arabidopsis Protein Phosphatase 2Cs1[W]
TLDR
It is found that the AHG3 gene is identical to AtPP2CA, which encodes a protein phosphatase 2C (PP2C), and it is demonstrated in vitro and in vivo that the ahg3-1 missense mutation causes the loss of PP1C activity, providing concrete confirmation that this PP2C functions as a negative regulator in ABA signaling.
The ABI1 and ABI2 protein phosphatases 2C act in a negative feedback regulatory loop of the abscisic acid signalling pathway.
TLDR
The isolation and characterisation of the first reduction-of-function allele of ABI2, abi2--1R1, indicate that the wild-type ABI 2 phosphatase is a negative regulator of ABA signalling, and that the ABI1 and A BI2 phosphatases have overlapping roles in controlling ABA action.
The Protein Phosphatase AtPP 2 CA Negatively Regulates Abscisic Acid Signal Transduction in Arabidopsis , and Effects of abh 1 on AtPP 2 CA mRNA 1 [ W ]
TLDR
The data demonstrate that the PP2CA protein phosphatase is a strong negative regulator of ABA signal transduction and down-regulated in the ABAhypersensitive nuclear mRNA cap-binding protein mutant abh1, and shows here that down-regulation of AtPP2CA in abh 1 is not due to impaired RNA splicing of At PP2 CA pre-mRNA.
ABI1 Protein Phosphatase 2C Is a Negative Regulator of Abscisic Acid Signaling
TLDR
Results indicate that a loss of ABI1 PP2C activity leads to an enhanced responsiveness to ABA, and that the wild-type ABA1 phosphatase is a negative regulator of ABA responses.
The Arabidopsis ABSCISIC ACID-INSENSITIVE2 (ABI2) and ABI1 genes encode homologous protein phosphatases 2C involved in abscisic acid signal transduction.
TLDR
The homologous ABI1 and ABI2 phosphatases appear to assume partially redundant functions in ABA signaling, which may provide a mechanism to maintain informational homeostasis.
The Protein Phosphatase AtPP2CA Negatively Regulates Abscisic Acid Signal Transduction in Arabidopsis, and Effects of abh1 on AtPP2CA mRNA1[W]
TLDR
The data demonstrate that the PP2CA protein phosphatase is a strong negative regulator of ABA signal transduction and down-regulation of AtPP2CA in abh1 is not due to impaired RNA splicing of Atpp2CA pre-mRNA.
Arabidopsis Basic Leucine Zipper Proteins That Mediate Stress-Responsive Abscisic Acid Signaling Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.010362.
TLDR
The results provide strong in vivo evidence that ABF3 and ABF4 mediate stress-responsive ABA signaling, and the temporal and spatial expression patterns of ABF 3 and ABf4 were consistent with their suggested roles.
ABA-Hypersensitive Germination1 encodes a protein phosphatase 2C, an essential component of abscisic acid signaling in Arabidopsis seed.
TLDR
Results suggest that AHG1 has specific functions in seed development and germination, shared partly with AHG3, and many seed-specific genes and ABA-inducible genes were highly expressed in ahg1-1 and ahg3-1 mutants compared with the wild-type.
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