Triggering of apoptosis by Puma is determined by the threshold set by prosurvival Bcl-2 family proteins.

@article{Callus2008TriggeringOA,
  title={Triggering of apoptosis by Puma is determined by the threshold set by prosurvival Bcl-2 family proteins.},
  author={Bernard Andrew Callus and Donia M Moujallad and John Silke and Robert E Gerl and Anissa M. Jabbour and Paul G Ekert and David L. Vaux},
  journal={Journal of molecular biology},
  year={2008},
  volume={384 2},
  pages={313-23}
}
Puma (p53 upregulated modulator of apoptosis) belongs to the BH3 (Bcl-2 homology 3)-only protein family of apoptotic regulators. Its expression is induced by various apoptotic stimuli, including irradiation and cytokine withdrawal. Using an inducible system to express Puma, we investigated the nature of Puma-induced apoptosis. In BaF(3) cells, expression of Puma caused rapid caspase-mediated cleavage of ICAD (inhibitor of caspase-activated deoxyribonuclease) and Mcl-1 (myeloid cell leukemia 1… CONTINUE READING