Trichomonas vaginalis kills and eats – evidence for phagocytic activity as a cytopathic effect

@article{Midlej2009TrichomonasVK,
  title={Trichomonas vaginalis kills and eats – evidence for phagocytic activity as a cytopathic effect},
  author={Victor Midlej and Marlene Benchimol},
  journal={Parasitology},
  year={2009},
  volume={137},
  pages={65 - 76}
}
SUMMARY This study reports that the cytopathic effect of Trichomonas vaginalis, an important human parasite of the urogenital tract, occurs due to mechanical stress and subsequent phagocytosis of the necrotic cells. The investigation was done using a primary culture of bovine oviduct epithelial cells (BOECs), grown either in monolayers or as floating cells. Trophozoites displaying different virulence levels were co-incubated with BOECs for times varying between 1 min and 48 h. Analyses were… Expand
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References

SHOWING 1-10 OF 53 REFERENCES
Trichomonas vaginalis: Ultrastructural Bases of the Cytopathic Effect
TLDR
Electron microscopy demonstrated a peculiar form of adhesion that appears to be specific for trichomonas, in which the basal surface of T. vaginalis formed slender channels through which microvilli and cytoplasmic fragments of epithelial cells were internalized. Expand
Contact-dependent cytopathogenic mechanisms of Trichomonas vaginalis
TLDR
Kinetic analysis of target cell killing by trichomonads revealed that the probability of CHO cell death was related to the likelihood of contact with T. vaginalis, supporting the observation by microscopy that trICHomonads kill cells only by direct contact. Expand
Trichomonas vaginalis: in vitro phagocytosis of lactobacilli, vaginal epithelial cells, leukocytes, and erythrocytes.
TLDR
Investigation of the interaction of two strains of Trichomonas vaginalis with the cell types present in the microenvironment of the parasite during human infections indicates that phagocytosis may be both an efficient means of obtaining nutrients for the parasite and a significant factor in the pathogenesis of trichomonal infections of the human genitourinary tract. Expand
Acanthamoeba castellanii: structural basis of the cytopathic mechanisms.
TLDR
Observations on the structural mechanisms of the cytopathic effect of Acanthamoeba castellanii trophozoites on cultured MDCK cell monolayer show lytic capacity of the amoebas appears to be the result of cytotoxic factors secreted by the ameebae since, when monolayers were incubated with conditioned medium, there was a decrease in the transepithelial resistance. Expand
An electron microscope study of the interaction between Trichomonas vaginalis and epithelial cells of the human amnion membrane
TLDR
Analysis of the electron microscope data showed that T. vaginalis established contact with the host cells as early as after 6 h of incubation; however, a close attachment of parasites to the epithelial cells occurred only after 9 h. Expand
Trichomonas adhere and phagocytose sperm cells: adhesion seems to be a prominent stage during interaction
TLDR
Differences in the behavior of both trichomonads were evident, showing that T. vaginalis was much more virulent than T. foetus when in interaction with bovine and human sperm cells. Expand
Pathogenic Trichomonas vaginalis cytotoxicity to cell culture monolayers.
TLDR
Release of tritiated thymidine (3H-thymidine) of the deoxyribonucleic acid (DNA) of prelabelled host cells after incubation with T vaginalis corroborated that extensive cytotoxicity was caused by pathogenic trichomonads in man. Expand
Structural bases of the cytolytic mechanisms of Entamoeba histolytica.
The cellular bases of the powerful cytolytic activity of the human protozoan parasite Entamoeba histolytica were explored by studying the effect of the virulent strain HM1:IMSS on epithelialExpand
Induction of human host cell apoptosis by Trichomonas vaginalis cysteine proteases is modulated by parasite exposure to iron.
TLDR
It is hypothesized that CP30 induction of host cell apoptosis is crucial for the development of a persistent infection, and that iron plays a determining role in parasite pathogenesis. Expand
Trichomonas vaginalis virulence against epithelial cells and morphological variability: the comparison between a well-established strain and a fresh isolate
TLDR
The FMVI strain of Trichomonas vaginalis was freshly isolated from an asymptomatic patient, and its morphological properties and virulence in vitro compared with the well-established JT strain did not correlate well with the clinical observations. Expand
...
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4
5
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