Trichome dynamics and artemisinin accumulation during development and senescence of Artemisia annua leaves.

  title={Trichome dynamics and artemisinin accumulation during development and senescence of Artemisia annua leaves.},
  author={W. Lommen and E. Schenk and H. Bouwmeester and F. Verstappen},
  journal={Planta medica},
  volume={72 4},
Artemisinin is a sesquiterpene lactone endoperoxide and an important antimalarial drug produced in Artemisia annua. To unravel the diverse processes determining artemisinin yield in A. annua crops, artemisinin accumulation during the development of individual leaves was studied in two field experiments. During the life cycle of a leaf, artemisinin was always present. Quantities were low at leaf appearance and increased steadily. In leaves studied until after senescence, maximum quantities and… Expand
Characterization of development and artemisinin biosynthesis in self-pollinated Artemisia annua plants
Artemisia annua L. is the only natural resource that produces artemisinin (Qinghaosu), an endoperoxide sesquiterpene lactone used in the artemisinin-combination therapy of malaria. TheExpand
Effects of N fertilization on trichome density, leaf size and artemisinin production in artemisia annua leaves
Results showed that effects of N fertilization were generally small, but in line with the hypothesis that at low N levels individual leaves remain smaller but have higher trichome densities, and these trends were especially clear in the branch leaves. Expand
Artemisinin production and precursor ratio in full grown Artemisia annua L. plants subjected to external stress
It is suggested that older and younger leaves may respond differently to applied stress, and changes in concentrations of AN-c seemed largely to be related to changes in GT densities and sizes. Expand
Differentially Expressed Genes during Contrasting Growth Stages of Artemisia annua for Artemisinin Content
The high utility small scale custom microarray chip of A. annua containing all the significant artemisinin biosynthesis-related genes, the established EST dataset, gene sequences isolated in-house and strategically selected candidates from theNCBI was employed to compare the gene expression profiles of two stages. Expand
Variations in key artemisinic and other metabolites throughout plant development in Artemisia annua L. for potential therapeutic use.
Results indicated that plants at the floral bud stage have the highest level of artemisinin and other therapeutic compounds for the treatment of malaria. Expand
Artemisinin Biosynthesis in Non-glandular Trichome Cells of Artemisia annua.
It is reported that non-GT cells of self-pollinated inbred A. annua plants can express the artemisinin biosynthetic pathway and leaves of regenerated gl plantlets biosynthesize artemis inin. Expand
Effect of roots on artemisinin and flavonoid production in shoots of Artemisia annua.
Artemisinin is a potent antimalarial sesquiterpene lactone produced and stored in the glandular trichomes (GLTs) of Artemisia annua. Although they produce no artemisinin, nor any of the precursorExpand
The effect of roots and media constituents on trichomes and artemisinin production in Artemisia annua L.
It is suggested that, while the presence of roots affects AN and trichome production, phytohormones and other media constituents used for in vitro culture of A. annua also exert an influence. Expand
Expression of β-glucosidase increases trichome density and artemisinin content in transgenic Artemisia annua plants.
Artemisinin content was increased with the expression of β-glucosidase (bgl1) gene in A. annua through Agrobacterium-mediated transformation and Artemisinin was enhanced up to five-fold in BGL1 transgenic flowers. Expand
Transgenic approach to increase artemisinin content in Artemisia annua L.
In this review, the progress on increasing artemisinin content in A. annua by transgenic approach and its future prospect are summarized and discussed. Expand