Tributyrate as a substrate for the determination of lipase activity in milk.

@article{Castberg1975TributyrateAA,
  title={Tributyrate as a substrate for the determination of lipase activity in milk.},
  author={H B Castberg and P C Solberg},
  journal={The Journal of dairy research},
  year={1975},
  volume={42 2},
  pages={247-53}
}
A procedure is described for the determination of the lipolytic activity in milk using tributyrate as substrate. The method is based on the titration of liberated butyric acid in a diethyl ether: light petroleum (2-75:1, v/v) extract of the incubation mixture. Some of the fundamental factors involved in the procedure are discussed.