Triazolam protein binding and correlation with alpha-1 acid glycoprotein concentration.

Abstract

On two occasions separated by a minimum of 1 wk, plasma was obtained from 12 patients (aged 18 to 73 yr) on dialysis after an overnight fast. Samples were assayed for albumin and alpha 1-acid glycoprotein (AGP) concentrations. 14C-Triazolam was added to each sample to a final concentration of 5 ng/ml. Protein binding was determined by equilibrium dialysis. Unbound triazolam ranged from 6.4% to 15.4% (mean = 10.0%). AGP concentrations ranged from 71.8 to 205.1 mg% (mean = 123.4 mg%). Triazolam binding ratio (bound/unbound concentration) correlated with AGP concentration (r2 = 0.69) but not with albumin concentration, age, or sex. This correlation was verified by adding AGP in varying amounts to control plasma.

Cite this paper

@article{Kroboth1984TriazolamPB, title={Triazolam protein binding and correlation with alpha-1 acid glycoprotein concentration.}, author={Patricia Dowley Kroboth and Randall B. Smith and Mia Sorkin and Marcia R Silver and Raymond Rault and Mary Garry and Randy P Juhl}, journal={Clinical pharmacology and therapeutics}, year={1984}, volume={36 3}, pages={379-83} }