Trehalose-mediated inhibition of the plasma membrane H+-ATPase from Kluyveromyces lactis: dependence on viscosity and temperature.

@article{Sampedro2002TrehalosemediatedIO,
  title={Trehalose-mediated inhibition of the plasma membrane H+-ATPase from Kluyveromyces lactis: dependence on viscosity and temperature.},
  author={Jos{\'e} G Sampedro and Rosario A. Mu{\~n}oz-Clares and Salvador Uribe},
  journal={Journal of bacteriology},
  year={2002},
  volume={184 16},
  pages={4384-91}
}
The effect of increasing trehalose concentrations on the kinetics of the plasma membrane H+-ATPase from Kluyveromyces lactis was studied at different temperatures. At 20 degrees C, increasing concentrations of trehalose (0.2 to 0.8 M) decreased V(max) and increased S(0.5) (substrate concentration when initial velocity equals 0.5 V(max)), mainly at high… CONTINUE READING