Trehalose-enhanced fluidity of the goat sperm membrane and its protection during freezing.

@article{Aboagla2003TrehaloseenhancedFO,
  title={Trehalose-enhanced fluidity of the goat sperm membrane and its protection during freezing.},
  author={Eiman M-E Aboagla and Takato N. Terada},
  journal={Biology of reproduction},
  year={2003},
  volume={69 4},
  pages={
          1245-50
        }
}
In an attempt to find a suitable freezing method for goat semen, two experiments were conducted to study the influence of trehalose on the cryopreservation of goat spermatozoa. In experiment 1, goat spermatozoa were frozen in trehalose extender (0.375 M) alone (100%) or at different combinations of trehalose with Tris-citric acid-glucose (TCG) extender (0%, 25%, 50%, 75%). Final concentrations of 20% (v:v) egg yolk and 4% (v:v) glycerol were employed in the extenders (osmolality = 370, pH = 7… CONTINUE READING

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