Trehalose Renders the Dauer Larva of Caenorhabditis elegans Resistant to Extreme Desiccation

  title={Trehalose Renders the Dauer Larva of Caenorhabditis elegans Resistant to Extreme Desiccation},
  author={Cihan Erkut and Sider Penkov and Hassan Khesbak and Daniela Vorkel and Jean-Marc Verbavatz and Karim Fahmy and Teymuras V. Kurzchalia},
  journal={Current Biology},

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Molecular Strategies of the Caenorhabditis elegans Dauer Larva to Survive Extreme Desiccation

These data show that the desiccation response is activated by hygrosensation (sensing the desiccative environment) via head neurons, which leads to elimination of reactive oxygen species and xenobiotics, expression of heat shock and intrinsically disordered proteins, polyamine utilization, and induction of fatty acid desaturation pathway.

The C. elegans dauer larva as a paradigm to study metabolic suppression and desiccation tolerance

The hypometabolic, stress-resistant dauer larva of Caenorhabditis elegans is used as an excellent model to study the molecular mechanisms of desiccation tolerance, such as maintenance of membrane organization, protein folding, xenobiotic and ROS detoxification in the dry state.

The role of phospholipid headgroup composition and trehalose in the desiccation tolerance of Caenorhabditis elegans.

The biological relevance of reducing the PC:PE ratio by PL headgroup adaptation should be the preservation of plasma membrane integrity by relieving mechanical strain from desiccated trehalose-containing cells during fast rehydration.

The glyoxylate shunt is essential for desiccation tolerance in C. elegans and budding yeast

The results reveal a novel physiological role for the glyoxylate shunt and elucidate a conserved metabolic rewiring that confers desiccation tolerance on organisms as diverse as worm and yeast.

C. elegans possess a general program to enter cryptobiosis that allows dauer larvae to survive different kinds of abiotic stress

It is demonstrated that another stress factor, high osmolarity, activates similar biochemical pathways that allow the preservation of cellular functionality in the absence of water and significantly increases the biosynthesis of glycerol making larva tolerant to freezing.

Trehalose metabolism genes of Aphelenchoides besseyi (Nematoda: Aphelenchoididae) in hypertonic osmotic pressure survival

Results indicated that trehalose metabolism genes should play a role in osmobiosis regulation and function within a restricted time frame.

Trehalose and tardigrade CAHS proteins work synergistically to promote desiccation tolerance

Beyond desiccation tolerance, this study provides insights into how the solution environment tunes intrinsically disordered proteins’ functions, many of which are vital in biological contexts such as development and disease that are concomitant with large changes in intracellular chemistry.

LEA motifs promote desiccation tolerance in vivo

The identification of LEA motifs that can function in both bacteria and in a multicellular organism in vivo suggests the possibility of engineering LEA-1-derived peptides for optimized desiccation protection.

Transcriptomic and proteomic analysis ofanhydrobiosis in Panagrolaimus superbusand Caenorhabditis elegans dauer larvae

The data presented in this thesis show that substantial reorganisation of cellular structures and mobilization of cellular protection repair systems occurs in C. elegans dauer larvae and P. superbus in response to desiccation, indicating that anhydrobiotic survival requires a major, integrated organismal response.



Maradolipids: diacyltrehalose glycolipids specific to dauer larva in Caenorhabditis elegans.

Dauer larvae obtained from starved plates of wild-type worms (N2) displayed a spot of comparable strength that represents a genuine lipid component of the natural dauer larvae, which does not depend on the genetic background or temperature.

Vitrification is essential for anhydrobiosis in an African chironomid, Polypedilum vanderplanki

Anhydrobiosis is an extremely dehydrated state in which organisms show no detectable metabolism but retain the ability to revive after rehydration. Thus far, two hypotheses have been proposed to

Intracellular trehalose is neither necessary nor sufficient for desiccation tolerance in yeast.

It is shown that there is no consistent relationship between intracellular trehalose levels and desiccation tolerance in S. cerevisiae, suggesting that other adaptations are more important.

Anhydrobiosis without trehalose in bdelloid rotifers

The role of trehalose in the physiology of nematodes.

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A gene in the anhydrobiotic nematode Aphelenchus avenae that is upregulated in response to desiccation stress and whose encoded protein shares sequence similarity with a late-embryonic gene that is induced in many plants when they are deprived of water is identified.

The anhydrobiotic potential and molecular phylogenetics of species and strains of Panagrolaimus (Nematoda, Panagrolaimidae)

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The role of vitrification in anhydrobiosis.

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Results obtained with infrared spectroscopy indicate that trehalose and DPPC interact by hydrogen bonding between the OH groups in the carbohydrate and the polar head groups of DPPC, and this interaction is specific totrehalose.

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A hypothetical scheme for the events of the induction of anhydrobiosis and recovery is presented and the proportion of animals recovering in water that will survive rapid dehydration decreases with the time they are in water.