Trehalase in the spermatophore from the bean-shaped accessory gland of the male mealworm beetle, Tenebrio molitor: purification, kinetic properties and localization of the enzyme

@article{Yaginuma1996TrehalaseIT,
  title={Trehalase in the spermatophore from the bean-shaped accessory gland of the male mealworm beetle, Tenebrio molitor: purification, kinetic properties and localization of the enzyme},
  author={Toshinobu Yaginuma and Takashi Mizuno and Chikaaki Mizuno and Mauro Kazuo Ikeda and Tsuyoshi Wada and Kenichi Hattori and Okitsugu Yamashita and George M. Happ},
  journal={Journal of Comparative Physiology B},
  year={1996},
  volume={166},
  pages={1-10}
}
Trehalase from the bean-shaped accessory glands of the male mealworm beetle, Tenebrio molitor, was purified by acid treatment, with subsequent chromatography on columns of DEAE-cellulofine and Sephacryl S-300. The molecular masses of the native and the denatured forms were estimated to be 43 and 62 kDa by gel filtration and SDS-PAGE, respectively, an indication that the trehalase may be composed of a single polypeptide. The optimum pH of the reaction catalyzed by trehalase was 5.6–5.8. The Km… CONTINUE READING
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