Trapping of nitric oxide produced during denitrification by extracellular hemoglobin.

@article{Goretski1988TrappingON,
  title={Trapping of nitric oxide produced during denitrification by extracellular hemoglobin.},
  author={J Goretski and Thomas C. Hollocher},
  journal={The Journal of biological chemistry},
  year={1988},
  volume={263 5},
  pages={2316-23}
}
A spectrophotometric method has been developed that uses extracellular hemoglobin (Hb) to trap nitric oxide (NO) released during denitrification as nitrosyl hemoglobin (HbNO). The rate of complexation of NO with Hb is about at the diffusion controlled limit for protein molecules and the product, HbNO, is essentially stable. Hb was added to an anaerobic bacterial suspension and denitrification was initiated with either KNO2 or KNO3. HbNO formation was observed for six species of denitrifying… CONTINUE READING
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