Trapping and visualization of a covalent enzyme–phosphate intermediate

@article{Murphy1997TrappingAV,
  title={Trapping and visualization of a covalent enzyme–phosphate intermediate},
  author={Jennifer E. Murphy and Boguslaw Stec and Lan Ma and Evan R. Kantrowitz},
  journal={Nature Structural Biology},
  year={1997},
  volume={4},
  pages={618-622}
}
Using a mutant version of E. coli alkaline phosphatase, we succeeded in trapping and determining the structure of the phospho-enzyme intermediate. The X-ray structure also revealed the catalytic water molecule, bound to one of the active site zinc ions, positioned ideally for the apical attack necessary for the hydrolysis of the intermediate. 
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