Trapped-oligonucleotide nucleotide incorporation (TONI) assay, a simple method for screening point mutations.

Abstract

We present a simple screening method for detecting a known point mutation, using only one 5'-biotinylated oligonucleotide primer, with its 3' end adjacent to the mutation site. In parallel reactions, an amplified DNA template encompassing the biotinylated oligonucleotide and mutation site undergoes 40 step-cycles of single nucleotide incorporation using Taq… (More)

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Cite this paper

@article{Prezant1992TrappedoligonucleotideNI, title={Trapped-oligonucleotide nucleotide incorporation (TONI) assay, a simple method for screening point mutations.}, author={T R Prezant and Nathan Fischel-Ghodsian}, journal={Human mutation}, year={1992}, volume={1 2}, pages={159-64} }