Transposon tagging in rice


To develop an efficient gene isolation method for rice we introduced the maize Ac/Ds system into rice. Extensive analysis of their behavior in rice for several generations indicated that Ac and Ds in the presence of Ac transposase gene actively transpose in rice. A wide spectrum of mutations affecting growth, morphogenesis, flowering time and disease resistance have been obtained in the population carrying Ac/Ds and some of them were genetically analyzed. Main efforts are currently being made to isolate genes responsible these mutations. In addition, a number of Ac/Ds were mapped on chromosomes and mapped elements will be used in the future for directed tagging of genes with known chromosomal positions.

DOI: 10.1023/A:1005769605026

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@article{Izawa1997TransposonTI, title={Transposon tagging in rice}, author={Takeshi Izawa and Tohru Ohnishi and Toshitsugu Nakano and Nobuhiro Ishida and Hiroyuki Enoki and Hisako Hashimoto and Kimiko Itoh and Rie Terada and Chuanyn Wu and Chikara Miyazaki and Tomoko Endo and Shigeru Iida and K . Shimamoto}, journal={Plant Molecular Biology}, year={1997}, volume={35}, pages={219-229} }