Transposon mutagenesis of the lyme disease agent Borrelia burgdorferi.

  title={Transposon mutagenesis of the lyme disease agent Borrelia burgdorferi.},
  author={Philip E Stewart and Patricia A Rosa},
  journal={Methods in molecular biology},
Borrelia burgdorferi, the causative agent of Lyme disease, is an obligate parasite that cycles between vertebrate hosts and tick vectors. Attempts to understand the genetic factors that allow B. burgdorferi to sense, adapt to, and survive in different environments have been limited by a relatively low transformation rate. Here, we describe a mariner-based transposon system that achieves saturating levels of random mutagenesis in B. burgdorferi. In comparison with allelic exchange, which targets… CONTINUE READING

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