Transposon mutagenesis of Bacillus anthracis strain Sterne using Bursa aurealis.

@article{Tam2006TransposonMO,
  title={Transposon mutagenesis of Bacillus anthracis strain Sterne using Bursa aurealis.},
  author={Christina P Tam and Elizabeth M. Glass and Deborah M. Anderson and Dominique Missiakas},
  journal={Plasmid},
  year={2006},
  volume={56 1},
  pages={
          74-7
        }
}
Bacillus anthracis, a spore forming Gram-positive microbe, is the causative agent of anthrax. Although plasmid encoded factors such as lethal toxin (LeTx), edema toxin (EdTx), and gamma-poly-d-glutamic acid (PGA) capsule are known to be required for disease pathogenesis, B. anthracis genes that enable spore invasion, phagosomal escape and macrophage replication are still unknown. To establish transposon mutagenesis as a tool for the characterization of anthrax genes, we employed the mariner… CONTINUE READING
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