Transposon-mediated enhancer trapping in medaka.

  title={Transposon-mediated enhancer trapping in medaka.},
  author={Clemens Grabher and Thorsten Henrich and Takao Sasado and Alexander Arenz and Joachim Wittbrodt and Makoto Furutani-Seiki},
We tested the Sleeping Beauty transposable element for its ability to efficiently insert transgenes into the genome of medaka (Oryzias latipes), an important model system for vertebrate development. We show that the SB transposon efficiently mediates integration of a reporter gene into the fish germ line. In pilot experiments, we established 174 transgenic lines with a transgenesis efficiency of 32%. Transgenes are stably transmitted to, and expressed in, subsequent generations. Interestingly… CONTINUE READING

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